Human Methylated & Non-Methylated (WGA) DNA Set
D5013 / D5013-1 / D5013-2
Human Methylated & Non-Methylated (WGA) DNA Set
Cat # | Name | Size | Price | Quantity |
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Documents

Highlights
- Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
- Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.
Description
Concentration | 250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
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Sample Source | Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase. |
Sample Storage | ≤ -20°C |
Q1: Are the Human methylated DNA standards completely (100%) methylated at the CpG sites?
All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).