Genomic DNA Clean & Concentrator-25

D4064 / D4065


Genomic DNA Clean & Concentrator-25

D4064 / D4065

Cat # Name Size Price Quantity
D4064 Genomic DNA Clean & Concentrator-25 25 Preps €91,00
+ -
D4065 Genomic DNA Clean & Concentrator-25 100 Preps €305,00
+ -

Documents


Genomic DNA Clean & Concentrator-25 (DCC) 25

Highlights


  • Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
  • Unique spin column for low volume elution of ultra-pure, high-yield DNA
  • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

Description


The Genomic DNA Clean & Concentrator-25 (DCC) is a spin column gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

Technical Specifications


Applicable For Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume ≥ 35 µl of DNA Elution Buffer
Equipment Microcentrifuge
Purity A260/A280 > 1.8, A260/A230 > 1.8
Sample Source Enzymatic reactions or impure preparations containing genomic DNAs
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Size Range 50 bp to 200 kb
Yield Recovery of DNA ranges from 70 - 95%

Product FAQ


Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Q2: How to process naked DNA stored in DNA/RNA Shield?

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Q5: How many times can columns be reloaded?

Q6: What is the minimum input volume of DNA sample?

Kit Components