DNase I Set

E1010


DNase I Set

E1010

Cat # Name Size Price Quantity
E1010 DNase I Set 250 U €56,00
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Documents


Datasheet: PDF   |   SDS (MSDS): PDF   |  
Enzyme for cutting both double-stranded and single-stranded DNA.

Highlights


  • Lyophilized enzyme provided with DNA Digestion Buffer

Description


DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The DNase I Set features lyophilized enzyme and DNA Digestion Buffer.

Technical Specifications


Concentration 1 U/µl
Heat inactivation Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min..
Included One unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). One unit of enzyme is equivalent to one Kunitz unit.
Resuspension Volume 275 µl
Size 250 U

Kit Components