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    DNase I Set (RNase-free)

    E1010 / E1011 / E1012

    Get Free Sample Limited time offer!

    DNase I Set (RNase-free)

    Get Free Sample Limited time offer!
    Cat # Name Size Price Quantity
    E1010 DNase I Set 250 U €75,50
    - +
    E1011 DNase I Set 1500 U €158,30
    - +
    E1012 DNase I Set 5 x 1500 U €710,20
    - +

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    • Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
    • RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
    • Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.

    Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.

    Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.

    1Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
    2Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
    3Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
    4Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.

    • Remove contaminating DNA from RNA or protein samples
    • Clean up of RNA samples prior to RT-PCR
    • DNA-free RNA sample preparation prior to RNA-seq
    • Remove DNA templates after in vitro transcription
    • DNA labeling by nick-translation
    • Reduce viscosity in cell culture or cell lysate
    • DNase I footprinting

    Cat # Name Size Price
    E1010-1-4 DNA Digestion Buffer 4 mL €17,40
    E1010-1-16 DNA Digestion Buffer 16 mL €33,50

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