|Applicable For||Next-Generation Sequencing, qPCR, microarray|
|Device Specs||Evacuated 16 mm x 100 mm blood collection tubes pre-filled with DNA/RNA Shield (6 mL)|
|Device Storage||Ambient temperature => 2 years|
|Sample Collection||3 mL blood draw|
|Sample Source||Whole blood (fresh or anti-coagulated)|
|Sample Stability||RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q3: How to collect solid tissues in DNA/RNA Shield?
Q4: Can I FACS sort directly into DNA/RNA Shield?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q7: What sample types is DNA/RNA Shield suitable for?
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Mouse blood in EDTA was stored in DNA/RNA Shield (2X concentrate) and genomic DNA was extracted for reduced representation bisulfite sequencing (RRBS). Researchers were able to show that mammalian DNA methylomes are characterized by CpG sites that can represent an organism’s biological age.Petkovich, DA et al. Using DNA Methylation Profiling to Evaluate Biological Age and Longevity Interventions. Cell Metabolism. 2017.
An RNA-based detection method was developed to detect low asymptomatic malaria infections in rural areas of Haiti. Three different detection methods were utilized--rapid diagnostic test, thick smear microscopy and a qRT-PCR assay were evaluated. The blood samples used for qRT-PCR were preserved in DNA/RNA Shield to maintain the sample integrity until RNA isolation. The qRT-PCR method was the most sensitive and identified significantly more samples.Elbadry MA, et. al. (2015). High prevalence of asymptomatic malaria infections: a cross‑sectional study in rural areas in six departments in Haiti. Malar. J. 14:510.
During a survey of Middle East respiratory syndrome coronavirus (MERS-CoV), nasal swabs from dromedary camels were collected into DNA/RNA Shield . Any MERS-positive swab samples were completely inactivated in DNA/RNA Shield and were subsequently used for RNA extraction. Successful detection of MERS-CoV and phylogenetic analysis suggests local zoonotic transmission through the respiratory route onto humans.Nowotny N, et. al. (2014). Middle east Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels, Oman, 2013. Eurosurveillance. 19(16).
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)