Bisulfite-Converted Universal Methylated Human DNA Standard
D5015
Bisulfite-Converted Universal Methylated Human DNA Standard
| Cat # | Name | Size | Price | Quantity |
|---|
Documents
Highlights
- DNA that has been enzymatically methylated at all cytosines within a CpG dinucleotide context and bisulfite-converted for direct use in downstream methylation applications.
- Provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
Description
| Concentration | 20ng/µl |
|---|---|
| Sample Storage | Store DNA standard at ≤ -20°C for up to 12 months. Prolonged storage should be ≤ -70°C. |
| Supplemental Info |
Q1: What is the source of the Universal Methylated Human DNA Standard (D5011) and Bisulfite-converted Universal Methylated Human DNA Standard (D5015)?
The sample is derived from human male brain tissue.
Q2: Are the Human methylated DNA standards completely (100%) methylated at the CpG sites?
All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).
Q3: How do you use this standard?
D5015 is fully methylated human DNA which has been bisulfite converted using the EZ DNA Methylation-Lightning Kit. When run in parallel with experimental samples through library prep, PCR, etc., this standard can be used to determine if there are issues with sample quality or conversion efficiency that may be negatively impacting downstream applications.
Q4: What is the primer set that is provided with the DNA standards? How do I use them?
This standard is included with primers targeting human MLH1 mismatch repair gene, and will amplify non-methylated, methylated, and mixed methylated templates. By amplifying bisulfite converted DNA with the included primers, researchers can test and optimize their bisulfite PCR conditions.
Q5: What polymerase do you suggest I use with these standards?
We recommend ZymoTaq DNA Polymerase, a hot start Taq polymerase specifically designed to amplify bisulfite converted DNA. Another hot start DNA polymerase of choice may also be used.
Q6: What is the stability of the standards?
The standards are stable at ≤ -20°C for up to 24 months. For long term storage standards should be stored at ≤ -70°C.