Quantifying Inflammatory Resolution in Menstrual Fluid

Menstruation is one of the few recurrent, non-pathological windows into inflammatory resolution in humans. Turning that into a quantitative platform meant solving sampling at scale, library chemistry for mixed host and microbial material, and analytics that explicitly model tissue composition and cycle timing rather than filtering them away.

• How NextGen Jane built and validated an ISO-aligned, at-home tampon collection kit that enables at-scale sampling.
• Why ribosomal-RNA depletion using Zymo's RiboFree dsDNA-nuclease chemistry is essential for simultaneous host and microbiome transcriptomics profiling.
• How modeling tissue composition and cycle timing turns menstrual fluid into a time-resolved profile of inflammation and repair.
• How inflammatory resolution fails differently in endometriosis and autoimmune disease.
• How this framework is opening new possibilities in non-invasive diagnostics in women's health.

Menstruation, properly modeled, becomes a quantitative human system for studying how inflammation starts and ends. That framing opens up inflammatory resolution as a measurable, dynamic process in humans, and reveals disease-specific failure modes that static snapshots of immune activation cannot see.