Quick-DNA Fecal/Soil Microbe 96 Magbead Kit

D6011-FM / D6010-FM / D6012-FM


Quick-DNA Fecal/Soil Microbe 96 Magbead Kit

D6011-FM / D6010-FM / D6012-FM

Cat # Name Size Price Quantity
D6011-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit 2 x 96 Preps €582,00
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D6010-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Rack) 2 x 96 Preps €852,00
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D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes) 2 x 96 Preps €852,00
+ -

Documents


Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.

Highlights


  • Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product.

Description


The Quick-DNA Fecal/Soil Microbe 96 Magbead Kits are fecal and soil DNA extraction kits designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 90 minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo MagBinding Bead technology, which features Zymo Research's Inhibitor Removal technology, is then used to isolate the DNA. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 37.5 µl
Equipment Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block, 2 mL 96 well plates and reagent carriers (user supplied).
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type Total DNA
Yield ≤ 25 µg total DNA

Product FAQ


Q1: Can I use more sample input by scaling up the protocol?

Q2: Do you have scripts available for your automated kits and/or do you provide scripting support?

Q3: Are there any tips in optimzing bead beating conditions?

Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q5: When can an RNase A treatment be implemented in the protocol?

Citations


Kit Components