The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
||Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
||≥ 10 µl of DNA Elution Buffer
||A260/A280 > 1.8, A260/A230 > 1.8
||Enzymatic reactions or impure preparations containing genomic DNA.
||Eluted DNA can be used immediately or stored at ≤ -20°C.
||50 bp to 200 kb
||Recovery of DNA ranges from 70 - 95%
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?