The Zymoprep Yeast Plasmid Miniprep kits are simple and efficient yeast plasmid miniprep products based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kits provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. The Zymoprep-96 incorporates our Zymopre II kit into a 96-well format resulting in a high-throughput solution that is ideal for screening multiple clones simultaneously. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.