Strep-Spin Protein Miniprep Kit

P2004 / P2005


Strep-Spin Protein Miniprep Kit

P2004 / P2005

Cat # Name Size Price Quantity
P2004 Strep-Spin Protein Miniprep Kit 10 Preps €81,00
+ -
P2005 Strep-Spin Protein Miniprep Kit 50 Preps €302,00
+ -

Documents


Fast protocol to purify Strep-tagged proteins from cell-free extracts.

Highlights


  • Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes.
  • Simple: Prepare pure protein for small-scale studies using a spin-column.
  • Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

Description


The Strep-Spin Protein Miniprep Kit™ provides a fast spin-column based purification technology for Strep-tagged proteins. Up to 450 µg of Strep-tagged protein can be purified in 7 minutes and eluted in Strep-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Technical Specifications


Affinity Matrix Strep-Tactin® XT Superflow® 50%
Binding Capacity 100 µl of Strep-Tactin® XT Superflow® 50% suspension can bind 15 nmol biotin (approx. 450 µg of a 30 kDa Twin-Strep-tag® protein).
Elution Method Biotin excess
Elution Volume Recommended 3 x 200 µl
Principle of Technology The Strep-Spin Protein Miniprep Kit is based on a novel StrepTactin® XT Superflow® resin which binds to Twin Strep-tag® with very high affinity. This tag contains two Strep-tag® II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of Twin-Step®-tagged proteins; however it will also work efficiently for proteins with single Strep-tags.
Processing Time 7 minutes
Product Storage Please store the Strep-Tactin® XT Superflow® 50% suspension, Strep-Wash Buffer, and Strep-Elution Bufer at 4°C. The other components can be stored at room temperature.
Protein Purity Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required Equipment Microcentrifuge
Sample Type Cell lysates or other complex protein mixtures containing Strep-tagged proteins.

Product FAQ


Q1: Which Step-Tag amino acid sequences are recommended?

Q2: Can the kit be used to purify membrane proteins?

Q3: Can the sample contain biotin?

Q4: Which pH should be used with the kit?

Q5: Which chemicals/reagents are compatible with the kit?

Q6: Is there a recommended lysis protocol?

Q7: How do you improve protein quality/purity?

Q8: Can less then 200 µl of Strep-Spin Elution Buffer be used for the elution step?

Q9: Is it necessary to equilibrate the resin?

Q10: What might reduce protein yields?

Q11: How do you purify insoluble proteins?

Kit Components