E2050 / E2051
- Ideal for robust, non-biased amplification of 5mC, 5hmC, and g5hmC-modified DNA.
- Premixed reagents for convenient reaction set-up.
- Compatible with a range of fluorescent dyes for use in real-time PCR.
QuestTaq PreMix is supplied as a convenient 2X concentrated master mix for robust PCR with little to no byproduct formation. It has been optimized for non-biased amplification of cystosine, 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and glucosyl-5-hydroxymethylcytosine (g5hmC) containing DNA. It features a unique DNA polymerase and optimized buffer system for amplification of DNA having modified/unmodified cytosines. QuestTaq DNA Polymerase has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.
|Concentration||Reaction conditions at 1X (20 µl total volume) will contain 2 units of QuestTaq DNA polymerase|
|Specific Activity||5’ – 3’ polymerization|
|Storage||QuestTaq PreMix should be stored between -20 C to -80 C for maximum performance. Performance is guaranteed for 1 year from the time of receipt.|
|Unit Definition||One unit (U) is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72C.|