This product has been discontinued. For any inquiries, please contact info@zymoresearch.com.

Quest 5-hmC Detection Kit (Discontinued)

D5410 / D5411 / D5415 / D5416


Quest 5-hmC Detection Kit (Discontinued)

D5410 / D5411 / D5415 / D5416

Cat # Name Size Price Quantity
D5410 Quest 5-hmC Detection Kit (Discontinued) 25 Preps discontinued
D5411 Quest 5-hmC Detection Kit (Discontinued) 50 Preps discontinued
D5415 Quest 5-hmC Detection-Lite Kit (Discontinued) 25 Preps discontinued
D5416 Quest 5-hmC Detection-Lite Kit (Discontinued) 50 Preps discontinued

Documents


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Highlights


  • Method to distinguish 5-hydroxymethylcytosine in sequence- and locus -specific context within DNA
  • DNA is eluted in water or low-salt buffer and is suitable for analysis by a variety of downstream applications
  • Includes Msp1 restriction enzyme

Description


The Quest 5-hmC Detection Kit allows for locus-specific detection of 5-hydroxymethylcytosine (5-hmC) using a simple and efficient reaction setup. Traditional methods cannot distinguish 5-hmC from 5-methylcytosine (5-mC). This kit features a robust and highly specific 5-hmC glucosyltransferase enzyme to specifically tag 5-hmC sites, yielding the modified base, glucosyl-5-hydroxymethylcytosine (g-5-hmC). After glucosylation of 5-hmC, digestion of DNA with Msp1 allow 5-hmC to be differentiated from 5-mC. Msp1 can efficiently digest DNA when a cytosine, 5-mC, or 5-hmC is present in their recognition site, but it is sensitive to the presence of g-5-hmC. By exploiting this sensitivity, the 5-hmC level of a specific locus can be interrogated by utilizing various downstream applications (e.g. endpoint PCR, qPCR, Next-Generation sequencing, etc.).

Technical Specifications


Additional Info Includes Msp1
Input High quality DNA suitable for use in restriction enzyme digestion. Require A260/280 > 1.8, A260/230 > 1.8.
Storage Store components at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage at -80C.

Citations


Kit Components