MBP-Spin Protein Miniprep Kit
P2006 / P2007
- Fast: Purify MBP-tagged proteins from cell lysates in less than 6 minutes.
- Simple: Prepare pure protein for small-scale studies using a spin-column.
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
The MBP-Spin Protein Miniprep Kit provides a fast spin-column based purification technology for MBP-tagged proteins. Up to 1000 µg of MBP-tagged protein can be purified in 6 minutes and eluted in MBP-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
|Affinity Matrix||Amylose Resin|
|Binding Capacity||100 µl of Amylose Resin typically binds ≥ 400 µg of fusion protein.|
|Elution Method||Excess Maltose|
|Elution Volume||≥ 200 µl|
|Principle of Technology||The MBP-Spin Protein Miniprep Kit uses an affinity matrix composed of amylose resin to specifically bind proteins fused to maltose-binding protein (MBP). MBP is known to significantly enhance the solubility of many proteins when fused to them.|
|Processing Time||6 minutes|
|Product Storage||Please store the Amylose Resin at 4 °C. The other components can be stored at room temperature.|
|Purity||Electrophoretically pure and suitable for enzyme kinetics, biochemical analyses, SDS-PAGE, and other applications.|
|Sample Type||Cell lysates or other complex protein mixtures containing MBP-tagged proteins.|