His-Spin Protein Miniprep

P2001 / P2002


His-Spin Protein Miniprep

P2001 / P2002

Cat # Name Size Price Quantity
P2001 His-Spin Protein Miniprep 10 Preps €81,00
+ -
P2002 His-Spin Protein Miniprep 50 Preps €302,00
+ -

Documents


Fast protocol to purify His-tagged proteins from cell-free extracts.

Highlights


  • Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.
  • Simple: Prepare pure protein for small-scale studies using a spin-column.
  • Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

Description


The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Technical Specifications


Affinity Matrix Nickel charged agarose
Binding Capacity 1 mg
Elution Method Imidazole gradient
Elution Volume 100 - 200 µl (150 µl is optimal)
Principle of Technology Based on a nickel-charged His-Affinity Gel (IMAC).
Processing Time 5 minutes
Product Storage Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
Protein Purity Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required Equipment Microcentrifuge
Sample Type Cell lysates or other complex protein mixtures containing His-tagged proteins.

Product FAQ


Q1: Is the product suitable for eukaryotic expression system (HEK cells)?

Q2: Can less than 100 µl of His-Elution Buffer be used for the elution step?

Q3: What might reduce protein yields?

Q4: Which chemicals/reagents are not compatible with the kit?

Q5: How do you purify insoluble proteins?

Q6: Can the kit be used to purify membrane proteins?

Q7: Which pH should be used with the kit?

Q8: How do you improve protein quality/purity?

Q9: Is there a recommended lysis protocol?

Q10: Which chemicals/reagents are compatible with the kit?

Citations


Kit Components