EZ DNA Methylation-Startup Kit
- The complete solution for bisulfite conversion. This all-in-one kit contains reagents for bisulfite conversion of DNA without the need for purification, methylated human DNA with control primers, and a robust hot-start PCR polymerase that is specifically formulated for bisulfite-converted DNA.
- Designed for the first-time user requiring a consolidated product to control for bisulfite conversion.
- Kit includes: one EZ DNA Methylation-Direct kit, one fully methylated Universal Methylated Human DNA Standard, and ZymoTaq DNA Polymerase.
The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA.
|Applicable For||Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.|
|Applications||Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.|
|Elution Volume||≥ 10 µl|
|Equipment||Thermocycler with heated lid and microcentrifuge.|
|Input||Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.|
|Processing Time||4 hours|
|Sample Source||Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.|
Q1: What leads to poor conversion efficiency/ low yields?
Q2: How to quantify / visualize converted DNA?
Q3: What is the minimum DNA size that can be recovered?
Q4: How long is bisulfite converted DNA stable at -20 °C?
Q5: Does bisulfite conversion only occur in a CpG context?
Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?
Q7: Which polymerase is recommended for amplification from bisulfite-converted DNA?
Q8: Tips for bisulfite primer design?
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