EZ-96 DNA Methylation-Gold MagPrep

D5042 / D5043


EZ-96 DNA Methylation-Gold MagPrep

D5042 / D5043

Cat # Name Size Price Quantity
D5042 EZ-96 DNA Methylation-Gold MagPrep 4 x 96 Rxns. €629,00
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D5043 EZ-96 DNA Methylation-Gold MagPrep 8 x 96 Rxns. €999,00
+ -

Documents


EZ-96 DNA Methylation-Gold MagPrep

Highlights


  • Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
  • A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.

Description


The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 500 pg - 2 µg of DNA.
Processing Time 3 hours
Recovery > 70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ


Q1: What leads to poor conversion efficiency/ low yields?

Q2: How to quantify / visualize converted DNA?

Q3: What is the minimum DNA size that can be recovered?

Q4: How long is bisulfite converted DNA stable at -20 °C?

Q5: Does bisulfite conversion only occur in a CpG context?

Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q7: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q8: Tips for bisulfite primer design?

Kit Components