|Applicable For||Next-Generation Sequencing, qPCR, microarray|
|Device Specs||Saliva collection tube; DNA/RNA Shield (2 mL)|
|Reagent Storage||Ambient temperature = > 2 years|
|Sample Collection||2 ml saliva collection|
|Sample Stability||RNA: Ambient temperature (4°C- 25°C) > 1 month
DNA: Ambient temperature (4°C- 25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Q1: How to collect solid tissues in DNA/RNA Shield?
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Q3: What sample types is DNA/RNA Shield suitable for?
Q4: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q8: Can I FACS sort directly into DNA/RNA Shield?
A robust and sensitive assay was developed to screen for the 18S rRNA gene of Plasmodium falciparum and Plasmodium vivax from asymptomatic and low density infections. The new RT-PCR based method could detect malaria infections that were significantly lower than the standard microscopy and rapid detection method. DNA/RNA Shield was used to preserve patient’s blood samples in the field for 14 days at 28°C and 80% humidity.Adams M, et. al. (2015). An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low‑density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples. Malar. J. 14:520.
The researchers sought to better understand the physiological importance of the non-sense-mediated mRNA decay (NMD) in murine Sertoli cells. Mice testes were preserved in DNA/RNA Shield and stored RNA isolation. The data revealed that the knockout of Upf2, which is part of the NMD pathway, resulted in testicular atrophy and sterility.Bao J, et. al. (2015). UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome. Development. 142: 352-362.
DNA/RNA Shield was used to store water collected onto filters and total RNA was isolated. A detailed metatranscriptomic analysis of the microbial communities points towards differing metabolic cycles occurring in anoxic and saline habitats in upper and deep sea regions respectively.Pachiadaki MG, et. al. (2014). Unveili
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)