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    DNA/RNA Shield Collection Tube w/ Swab Sample


    DNA/RNA Shield Collection Tube w/ Swab Sample

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    Description

    Swab collection kits include DNA/RNA Shield Collection tubes with Swabs and are a general purpose collection method for molecular-based applications. Many sample types can be collected with these swab collection tubes including from the mouth, nose, throat, and wherever a swab may be deemed appropriate per application. To use these swab collection tubes, just swab any sample and break the tip into the collection device prefilled with the DNA/RNA Shield reagent. Samples are stabilized and ready for transport or storage.

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    Applicable For Next-Generation Sequencing, qPCR, microarray
    Device Specs 12 x 80 mm screwcap vial pre-filled with DNA/RNA Shield (1 mL or 2 mL)
    Device Storage Ambient temperature => 2 years
    Sample Collection 10% (v/v or w/v)
    Sample Source Nose, mouth, throat, biological fluids, environmental samples
    Sample Stability RNA: Ambient temperature (4°C-25°C) > 1 month
    DNA: Ambient temperature (4°C-25°C) > 2 years
    DNA & RNA: Frozen (<-20°C): Indefinitely

    Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.

    Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).

    DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).

    RNA > 1 month
    DNA > 2 years

    DNA & RNA (<-20°C): Indefinitely

    Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.

    No, tissues do not need to be homogenized.

    Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).




    “I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”

    - Laura T. (USDA)

    “We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”

    - Umberto P. (University of Pavia)

    “I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”

    - Maha AE. (University of Florida)

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