Alpha-Factor Mating Pheromone

Y1001


Alpha-Factor Mating Pheromone

Y1001

Cat # Name Size Price Quantity
Y1001 Alpha-Factor Mating Pheromone 240 µl €145,00
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Documents


Aqueous solution for arresting yeast in G1 of the cell cycle

Highlights


  • Aqueous Solution: Convenient ready-to-use solution of yeast alpha-factor mating pheromone for mating induction and G1 cell cycle arrest.
  • Robust and Efficient: Liquid solution has been optimized for both activity and stability and is guaranteed to retain biological function through multiple freeze-thaw cycles.
  • Easy: Widely-used simple method for studying the cell cycle, cellular morphology, transcriptional induction, and signal transduction pathways.

Description


When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed "schmooing". These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a/alpha diploids are not responsive to mating pheromone of either type, but can be induced to undergo meiosis via nutrient deprivation. The use of yeast mating pheromones has pioneered the study of the cell cycle, cellular morphology, transcriptional induction, as well as signal transduction pathways. Zymo Research provides the alpha-factor peptide mating pheromone as a ready to use liquid that has been optimized for both activity and stability and is guaranteed to retain biological function through multiple freeze-thaw cycles.

Technical Specifications


Amino Acid Sequence TRP-HIS-TRP-LEU-GLN-LEU-LYS-PRO-GLY-GLN-PRO-MET-TYR
Concentration 10 mM in 0.1 M sodium acetate pH 5.2, (i.e., 4 mg/240 µl).
Molecular Weight 1684.0 Da
Product Storage Store at -20°C. Alpha-factor is stable during multiple freeze-thaw cycles.
Purity > 98% by HPLC
Usage Recommended working concentration: ~5 µM (bar1Δ) to 100 µM (BAR1).