Considerations for Building an Accurate Shotgun Metagenomic Workflow for Gut Microbiome Profiling

Learn about the six major steps for using shotgun metagenomic sequencing to analyze the human gut microbiome, from sample collection through bioinformatic analysis. Understand the various biases that can arise in each step and become familiar with the workflow that has been optimized to generate accurate and reproducible results.

Highlights:

• Workflow breakdown including cost estimations
• Key challenges and major considerations
• Recommended methods based on comparison data of real samples and quality control standards