E. coli Overview
Despite the remarkable diversity of research interests in labs throughout the world, most researchers use E. coli transformation as a means for cloning and protein purification. With the needs of the researcher in mind, Zymo Research has developed a range of premade, chemically competent E. coli strains for high efficiency transformation (> 108 transformants/μg pUC19 DNA). These innovative Mix & Go Competent Cells feature a streamlined transformation procedure that eliminates heat shock, long incubations, and the need for electroporation. With the chemically competent Mix & Go E. coli from Zymo Research, a scientist can transform cells in less than 20 seconds… Simply mix and plate!
Zymo Research also provides the reagents so researchers can make their own Mix & Go Competent E. coli from their favorite lab strains. Included is a specially formulated growth medium, ZymoBroth™, which enhances the transformation efficiencies of many K- and B-strains of E. coli. Increase transformation efficiency and decrease transformation time with the Mix & Go system!